Journal: eLife

Article Title: CPT1A mediates radiation sensitivity in colorectal cancer

doi: 10.7554/eLife.97827

Figure Lengend Snippet: ( A ) The protein level of FOXM1, CPT1A, catalase (CAT), SOD1, SOD2, SOD3 after knockout and overexpression of CPT1A. ( B ) The mRNA level of FOXM1, CAT, SOD1, SOD2, SOD3 after knockout and overexpression of CPT1A. ( C ) Venn diagram showing the potential transcription factor of SOD1, SOD2, and CAT. ( D ) The protein level of FOXM1, CPT1A, CAT, SOD1, SOD2 after overexpression of FOXM1 in HCT116-CPT1AKO cells. ( E ) Schematic diagram summarising our working model, namely, decreased CPT1A promotes the transcription factor activity of FOXM1, increasing the mRNA and protein level of CAT, SOD1, and SOD2, followed by increasing ROS scavenge after irradiation and therefore colorectal cancer (CRC) cells become radioresistance. ***p<0.001, **p<0.01, *p<0.05. Figure 6—source data 1. Original files for western blot analysis displayed in . Figure 6—source data 2. PDF file containing original western blots for .

Article Snippet: Antibody , SOD3 Rabbit Polyclonal antibody , Proteintech (China) , 14316-1-AP , .

Techniques: Knock-Out, Over Expression, Activity Assay, Irradiation, Western Blot

Journal: eLife

Article Title: CPT1A mediates radiation sensitivity in colorectal cancer

doi: 10.7554/eLife.97827

Figure Lengend Snippet:

Article Snippet: Antibody , SOD3 Rabbit Polyclonal antibody , Proteintech (China) , 14316-1-AP , .

Techniques: Transfection, Construct, SYBR Green Assay, Protein Extraction, Single Cell Gel Electrophoresis, Activity Assay

Journal: Cell Death Discovery

Article Title: Regulation of cardiac ferroptosis in diabetic human heart failure: uncovering molecular pathways and key targets

doi: 10.1038/s41420-024-02044-w

Figure Lengend Snippet: Immunoblotting shows downregulation of cardiac SOD3 ( A ) and upregulation of MMP9 ( B ) in diabetic heart failure. Downregulated SOD3 and upregulated MMP9 are molecular markers of heart failure. Unpaired two-tailed t-test. * P < 0.05; *** P < 0.001. Each point represents one patient. N = 4–5. SOD3 = superoxide dismutase-3, MMP9 = matrix metalloproteinase-9, CT = control, healthy subjects, dHF = diabetic heart failure patients.

Article Snippet: The secondary antibody incubation was conducted at room temperature for 1 h. The antibodies used for the western blot detection were as follows: GPX4 (ab125066), ATF4 (10835-1-AP), GSR (18257-1-AP), NRF2 (16396-1-AP), TFR (17435-1-AP), STEAP3 (Thermo Fisher PA5-20406), DMT1 (20507-1-AP), FLC (10727-1-AP), FPN1 (26601-1-AP), APP (14-9749-82), HMOX1 (10701-1-AP), LPCAT3 (67882-1-Ig), ACOT1 (ab133948), SOD3 (14316-1-AP), MMP9 (10375-2-AP), anti-4-hydroxynonenal antibody (ab46545), caspase 3 (CST: #9662), caspase 1 (CST: #2225), caspase 8 antibody (ab25901), GSDMD (ProteinTech 66387-1-Ig), and MLKL (ProteinTech 66675-1-Ig).

Techniques: Western Blot, Two Tailed Test, Control

Journal: Cell Death Discovery

Article Title: Regulation of cardiac ferroptosis in diabetic human heart failure: uncovering molecular pathways and key targets

doi: 10.1038/s41420-024-02044-w

Figure Lengend Snippet: Immunoblotting shows downregulation of cardiac SOD3 ( A ) and upregulation of MMP9 ( B ) in diabetic heart failure. Downregulated SOD3 and upregulated MMP9 are molecular markers of heart failure. Unpaired two-tailed t-test. * P < 0.05; *** P < 0.001. Each point represents one patient. N = 4–5. SOD3 = superoxide dismutase-3, MMP9 = matrix metalloproteinase-9, CT = control, healthy subjects, dHF = diabetic heart failure patients.

Article Snippet: The secondary antibody incubation was conducted at room temperature for 1 h. The antibodies used for the western blot detection were as follows: GPX4 (ab125066), ATF4 (10835-1-AP), GSR (18257-1-AP), NRF2 (16396-1-AP), TFR (17435-1-AP), STEAP3 (Thermo Fisher PA5-20406), DMT1 (20507-1-AP), FLC (10727-1-AP), FPN1 (26601-1-AP), APP (14-9749-82), HMOX1 (10701-1-AP), LPCAT3 (67882-1-Ig), ACOT1 (ab133948), SOD3 (14316-1-AP), MMP9 (10375-2-AP), anti-4-hydroxynonenal antibody (ab46545), caspase 3 (CST: #9662), caspase 1 (CST: #2225), caspase 8 antibody (ab25901), GSDMD (ProteinTech 66387-1-Ig), and MLKL (ProteinTech 66675-1-Ig).

Techniques: Western Blot, Two Tailed Test, Control

Journal: World Journal of Gastroenterology

Article Title: N-linked glycoproteomic profiling in esophageal squamous cell carcinoma

doi: 10.3748/wjg.v28.i29.3869

Figure Lengend Snippet: Western blot validations of potential glycoprotein biomarkers. A and B: Representative Western blot results show haptoglobin (HP) (A) and procathepsin D (pCD) (B) with differential expression between esophageal squamous cell carcinoma (ESCC/T) and adjacent non-cancerous tissues (N); C: Representative Western blot results show the N-linked glycosylated fraction of HP, pCD, clusterin, superoxide dismutase 3 (SOD3), proline-arginine-rich end leucine-rich repeat protein (PRELP), and 14-3-3ζ in ESCC/T and N enriched by corresponding lectins; D: Representative Western blot results show the N-linked glycosylated fractions of clusterin, PRELP, and HP in serum of patients with ESCC/T and healthy controls. The results are representative of three independent experiments. ESCC/T: Esophageal squamous cell carcinoma; HP: Haptoglobin; pCD: Procathepsin D; SOD3: Superoxide dismutase 3; PRELP: Proline-arginine-rich end leucine-rich repeat protein.

Article Snippet: The antibodies used in this study were: Haptoglobin (1:2000, 16665-1-AP, Proteintech), cathepsin D (1:2500, ab75852, Abcam), clusterin (1:5000, 12289-1-AP, Proteintech), SOD3 (1:1500, T1799, Epitomics), PRELP (1:2000, AP6665b, Abgent), and 14-3-3-ζ (1:2500, ab85268, Abcam).

Techniques: Western Blot, Expressing

Journal: Frontiers in Pharmacology

Article Title: Estrogen-Related Receptor γ Agonist DY131 Ameliorates Lipopolysaccharide-Induced Acute Liver Injury

doi: 10.3389/fphar.2021.626166

Figure Lengend Snippet: Primer sequence.

Article Snippet: Immunoblotting was performed using primary antibodies against ERRγ (Santa Cruz, sc-66883, 1:1,000), β-actin (Bioss, bs-0061R, 1:2,500), SOD1 (Proteintech, 67480-1-Ig, 1:5,000), SOD2 (Abclonal, A19576, 1:1,000), SOD3 (Abclonal, A6984, 1:1,000), cleaved caspase-3 (Cell Signaling Technology, 9,661, 1:1,000), and Bax (Cell Signaling Technology, 2,772, 1:1,000), followed by the incubation of peroxidase-conjugated goat anti-rabbit secondary antibody (Beyotime, A0208, 1:2,000).

Techniques: Sequencing

Journal: Frontiers in Pharmacology

Article Title: Estrogen-Related Receptor γ Agonist DY131 Ameliorates Lipopolysaccharide-Induced Acute Liver Injury

doi: 10.3389/fphar.2021.626166

Figure Lengend Snippet: DY131 ameliorated oxidative stress in LPS-treated mice. (A,B) The levels of the antioxidant GSH and oxidative stress marker MDA in livers were measured using a commercial kit (n = 10 in each group). (C) mRNA expressions of SOD1, SOD2, and SOD3 were determined by qRT-PCR (n = 10 in each group). (D) Protein levels of SOD1, SOD2, and SOD3 were detected by Western blotting (n = 8 in each group). (E) Quantitative analyses of SOD1, SOD2, and SOD3 by densitometry (n = 8 in each group). (F) Representative images of DHE staining of liver tissues in different groups (magnification ×200, scale bar: 50 μm, n = 3 in each group). (G) Quantification of the mean fluorescence intensity of DHE was analyzed by ImageJ software. Data were presented as means ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001 vs. the indicated group. NS, no significance.

Article Snippet: Immunoblotting was performed using primary antibodies against ERRγ (Santa Cruz, sc-66883, 1:1,000), β-actin (Bioss, bs-0061R, 1:2,500), SOD1 (Proteintech, 67480-1-Ig, 1:5,000), SOD2 (Abclonal, A19576, 1:1,000), SOD3 (Abclonal, A6984, 1:1,000), cleaved caspase-3 (Cell Signaling Technology, 9,661, 1:1,000), and Bax (Cell Signaling Technology, 2,772, 1:1,000), followed by the incubation of peroxidase-conjugated goat anti-rabbit secondary antibody (Beyotime, A0208, 1:2,000).

Techniques: Marker, Quantitative RT-PCR, Western Blot, Staining, Fluorescence, Software

Journal: International Journal of Molecular Sciences

Article Title: Anakinra Activates Superoxide Dismutase 2 to Mitigate Inflammasome Activity

doi: 10.3390/ijms22126531

Figure Lengend Snippet: Anakinra spares mitochondria from oxidative damage by inducing PGC1α and SOD2: ( a ) Scanning electron microscopy of RAW 264.7 cells exposed to anakinra for 4 h. Images were taken with the Philips XL30 field emission scanning electron microscope. Note the presence of autophagic vacuoles engulfing enlarged mitochondria (arrows in the inset) in untreated cells as opposed to intact mitochondria (arrows in the inset) in anakinra-treated cells (indicated by squares and magnified in the insets). ( b ) Expression of the peroxisome proliferator-activated receptor coactivator 1α ( Pgc1α ) by RT-PCR in alveolar macrophages exposed to anakinra in the presence or not of Aspergillus conidia. ( c – g ) RAW 264.7 cells were treated with anakinra ( c – g ) and/or Aspergillus conidia ( d , g ) for 4 h ( d , f , g ) or the indicated times ( c , e ) for the expression of SOD2 ( c , d ), SOD1 and SOD3 ( e ), catalase activity ( f ) and the expression of TXN2 ( g ). PMA: Phorbol 12-myristate 13-acetate; DPI: Diphenyleneiodonium. * p < 0.05, ** p < 0.01, two-way ANOVA, none vs. anakinra.

Article Snippet: Blots of cell lysates were incubated with antibodies against the following proteins: COPS3, Abcam, Cat. No. Ab79698; SOD1 (Novus Biologicals); SOD2, Millipore, Cat. No. 06-984; SOD2, Thermo Fisher Scientific, Cat. No. A21990, Clone 9E2BD2; SOD3: Abcam, Cat. No. Ab83108; TXN2 (Novus Biologicals); USP36, Proteintech, Cat. No. 14783-1-AP.

Techniques: Electron Microscopy, Microscopy, Expressing, Reverse Transcription Polymerase Chain Reaction, Activity Assay

Journal: International Journal of Molecular Sciences

Article Title: Anakinra Activates Superoxide Dismutase 2 to Mitigate Inflammasome Activity

doi: 10.3390/ijms22126531

Figure Lengend Snippet: Anakinra promotes the interaction between SOD2, USP36, and COP9 signalosome: ( a ) Western blot analyses of RAW 264.7 cells exposed or not (none) to 10 μg/mL anakinra in the presence of cyclohexamide (CHX) and evaluated at the indicated times for SOD2 protein level. Relative densitometric evaluation was reported. ( b ) RAW 264.7 cell lysates obtained at 0, 2 and 4 h following anakinra exposure were immunoprecipitated using anti-SOD2 antibody and subsequently revealed for binding to the specific deubiquitinating protein USP36. SOD2 was used as IP control. ( c ) Cell lysates from RAW 264.7 cells treated with anakinra were immunoprecipitated using COPS3 antibody and were revealed for binding to both USP36 and SOD2. COPS3 was used as IP control. WCL: whole cell lysate; IP: immunoprecipitation.

Article Snippet: Blots of cell lysates were incubated with antibodies against the following proteins: COPS3, Abcam, Cat. No. Ab79698; SOD1 (Novus Biologicals); SOD2, Millipore, Cat. No. 06-984; SOD2, Thermo Fisher Scientific, Cat. No. A21990, Clone 9E2BD2; SOD3: Abcam, Cat. No. Ab83108; TXN2 (Novus Biologicals); USP36, Proteintech, Cat. No. 14783-1-AP.

Techniques: Western Blot, Immunoprecipitation, Binding Assay

Journal: International Journal of Molecular Sciences

Article Title: Anakinra Activates Superoxide Dismutase 2 to Mitigate Inflammasome Activity

doi: 10.3390/ijms22126531

Figure Lengend Snippet: Anakinra and SOD2 ameliorates the inflammatory pathology in vivo: ( a ) C57BL/6 mice were treated with rotenone in the presence or absence of anakinra and evaluated for lung histopathology. ( b – d ) C57BL/6 mice were infected intranasally with live A. fumigatus conidia and treated with Si Sod2 or equivalent doses of nonspecific, scrambled SiRNA in a volume of 20 µl of duplex buffer before evaluation of fungal growth (log10 cfu mean ± SEM) ( b ), lung histopathology ( c ) and cytokine quantification ( d ) at 3 dpi. Scale bar, 500 μm. **, p < 0.01; ***, p < 0.001, one-way ANOVA.

Article Snippet: Blots of cell lysates were incubated with antibodies against the following proteins: COPS3, Abcam, Cat. No. Ab79698; SOD1 (Novus Biologicals); SOD2, Millipore, Cat. No. 06-984; SOD2, Thermo Fisher Scientific, Cat. No. A21990, Clone 9E2BD2; SOD3: Abcam, Cat. No. Ab83108; TXN2 (Novus Biologicals); USP36, Proteintech, Cat. No. 14783-1-AP.

Techniques: In Vivo, Histopathology, Infection

Journal: International Journal of Molecular Sciences

Article Title: Anakinra Activates Superoxide Dismutase 2 to Mitigate Inflammasome Activity

doi: 10.3390/ijms22126531

Figure Lengend Snippet: Anakinra increased the lung expression of SOD2 in vivo: C57BL/6 ( a , b ), p47 phox−/− ( a ) and Cftr F508del ( b ) mice were infected with live Aspergillus conidia and treated with 10 mg/kg anakinra intraperitoneally for 6 consecutive days before analysis of SOD2 protein expression by immunofluorescence. Scale bar, 100 μm. Naïve: untreated mice, None: mice infected with Aspergillus conidia; anakinra: mice infected with Aspergillus conidia and treated with anakinra.

Article Snippet: Blots of cell lysates were incubated with antibodies against the following proteins: COPS3, Abcam, Cat. No. Ab79698; SOD1 (Novus Biologicals); SOD2, Millipore, Cat. No. 06-984; SOD2, Thermo Fisher Scientific, Cat. No. A21990, Clone 9E2BD2; SOD3: Abcam, Cat. No. Ab83108; TXN2 (Novus Biologicals); USP36, Proteintech, Cat. No. 14783-1-AP.

Techniques: Expressing, In Vivo, Infection, Immunofluorescence